CD40 Ligand 抗体
Quick Overview for CD40 Ligand 抗体 (ABIN118902)
抗原
See all CD40 Ligand (CD40LG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises the CD154 cell surface antigen, a 32 kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells. This antibody binds to CD154 at an epitope distinct from the CD40 binding site.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Mouse myeloma cell line transfected with human CD40L (CD154). Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63.Ag 8653 myeloma cell line.
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亚型
- IgG1
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anti-CD40 Ligand (CD40LG) (AA 1-261) antibody
CD40LG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 108-261) antibodyCD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2761 unconjugated
anti-CD40 Ligand (CD40LG) (AA 108-261) antibodyCD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2763 unconjugated
anti-CD40 Ligand (CD40LG) antibody (FITC)CD40LG 适用: 小鼠 FACS 宿主: 仓鼠 Monoclonal MR-1 FITC
anti-CD40 Ligand (CD40LG) (AA 33-62) antibodyCD40LG 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 111-261) antibodyCD40LG 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) antibody (PE)CD40LG 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 24-31 PE
anti-CD40 Ligand (CD40LG) (AA 47-261) antibodyCD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5A3A9 unconjugated
anti-CD40 Ligand (CD40LG) (AA 47-261) antibodyCD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8H10F5 unconjugated
anti-CD40 Ligand (CD40LG) (AA 47-65), (N-Term) antibodyCD40LG 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2, 0.09 % Sodium Azide, 5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD40 Ligand (CD40LG)
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别名
- CD154 / CD40L
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背景
- CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes. The protein encoded by this gene is a member of the TNF receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
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基因ID
- 959
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NCBI登录号
- NP_000065
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UniProt
- P29965
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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